Plant Transcription Factor Database
Previous version: v3.0
Azadirachta indica
HSF Family
Species TF ID Description
Neem_10058_f_3HSF family protein
Neem_10257_f_1HSF family protein
Neem_11145_a_3HSF family protein
Neem_13053_f_1HSF family protein
Neem_13333_f_3HSF family protein
Neem_14100_f_1HSF family protein
Neem_14329_f_1HSF family protein
Neem_1512_f_4HSF family protein
Neem_19563_f_1HSF family protein
Neem_22125_f_2HSF family protein
Neem_22908_f_1HSF family protein
Neem_23732_f_1HSF family protein
Neem_24176_f_2HSF family protein
Neem_24692_f_2HSF family protein
Neem_24864_a_2HSF family protein
Neem_25391_f_1HSF family protein
Neem_2547_f_4HSF family protein
Neem_29976_a_1HSF family protein
Neem_3096_f_1HSF family protein
Neem_4529_f_2HSF family protein
Neem_524_f_1HSF family protein
Neem_5997_f_2HSF family protein
Neem_6387_f_5HSF family protein
Neem_6438_f_1HSF family protein
Neem_6837_f_2HSF family protein
Neem_7649_f_2HSF family protein
Neem_9045_f_2HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645